With extensive experience in aptamer development, our scientists are confident in providing a full range of aptamer screening services to advance science and technology development. Creative Biolabs is able to provide aptamer screening services for multiple targets in an efficient and high-quality manner.
Most of the early aptamer screens were for purified proteins, but for some purified proteins, such as transmembrane proteins, ion channel proteins, etc. Aptamer screening for proteins is not of obvious significance. Moreover, in many cases, the object of study is not a single protein, but a complex composed of multiple proteins, such as cells, bacteria.
The difference between multi-target screening and conventional screening is only in the composition of the target, and the screening process is not significantly different from the latter. However, due to the complex composition of the target, it is more difficult to obtain aptamers by conventional screening methods. Therefore, the conventional SELEX technique needs to be optimized to achieve aptamer screening.
| Target Expressed on Cell Surface SELEX | The membrane protein is expressed on the cell surface by genetic engineering, and then the aptamer is obtained by complex target screening. This technology has great potential for application. |
| Toggle SELEX | A method for screening against multiple targets simultaneously. The screening cycle is switched between different targets according to different strategies. In this way, it is possible to diversify the functions of the same aptamer and to avoid technical difficulties such as purification of the target protein. |
| Counter SELEX | Cell-SELEX is a systematic aptamer screening process commonly used to screen for target proteins on cell membranes. However, the Cell-SELEX procedure still has some drawbacks, such as the interference of dead cells during the selection process and the lengthy experimental procedure. Counter-SELEX is a new negative selection procedure that can effectively eliminate oligomers from surface molecules, often in combination with cell SELEX. |
| Spiegemer SELEX | Mirror screening is an aptamer screening using the spin properties of chiral molecules. A library of right-handed oligonucleotides is used to screen for the spin isomers of the target. The obtained aptamer is synthesized as a levorotatory structure. Such aptamers not only recognize the target molecule, but also have high stability due to their left-handed structure which is not easily recognized by ribonucleases. |
| Complex Targets SELEX | The same oligonucleotide library was used to simultaneously screen for specific aptamers of different targets in the mixture without interfering with each other. |
| Subtractive SELEX | Using the filtered secondary library, specific aptamers for differential target molecules can be obtained in two highly homologous mixtures of intact cells. |
With our technology platform, Creative Biolabs provides high-quality aptamer screening services for multiple targets.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry.
