With extensive experience in aptamer development, our scientists are confident in providing a full range of toggle SELEX services to advance science and technology development. Creative Biolabs is able to provide toggle SELEX services in an efficient and high-quality manner.
Toggle SELEX is a modified SELEX technique established in 2001. The technique applies target proteins of different species and is able to screen for species cross-reacting aptamer.
| Clinical Drug Development | Structural Domain-Specific Apatmer |
|---|---|
| Since clinical drug development often applies human proteins as targets, but animal models are used for activity assays and preclinical experiments, this aptamer, which is active against both human and animal targets, facilitates preclinical evaluation of drug molecules on animal models. | For a protein, a conserved structural domain of the species is often required for the protein to function. Toggle SELEX to obtain apatmer specific to this structural domain can improve the functional activity of aptamer. |
| Aptamer Screening of Receptor Family proteins | Aptamer Screening for Homogeneous Molecules |
| Toggle SELEX is also suitable for screening receptor family proteins, ligand family proteins or structure-related protein targets with redundant or overlapping functions within the same species. | Toggle SELEX can also be applied to aptamer screening of similar small molecule targets, screening with molecules containing the same functional group and those without functional groups as targets. |
Researchers have screened a mixture of human and porcine thrombin by toggle SELEX application random RNA library, and then aptamer was obtained that specifically bound to both human and porcine thrombin. Researchers used gentamicin and apomycin, kanamycin and tobramycin, balomycin and neomycin, and streptomycin and dihydrostreptomycin in four groups. The RNA nucleic acid aptamers recognizing the aminoglycoside antibiotics were obtained by switching screening using four groups of aminoglycoside antibiotics.
Fig 1. Process of toggle SELEX [1].
With our technology platform, Creative Biolabs provides high-quality aptamer screening services by toggle SELEX.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs provides the most comprehensive services for aptamer development projects. Our cutting-edge technology allows our researchers to fulfill your requirement from various aspects and to offer the most suitable solution for your project.
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