With extensive experience in aptamer development, our scientists are confident in providing a full range of subtractive SELEX services to advance science and technology development. Creative Biolabs is able to provide subtractive SELEX services in an efficient and high-quality manner.
Since the development of SELEX technology, a variety of improved technical methods have emerged. Automation of the screening process, diversification of screening forms, and rapid screening results have been achieved.
In order to eliminate the nonspecific effect in aptamer screening, tRNA or affinity matrix is often used as the nonspecific target for reverse screening, which is called subtractive SELEX. In brief, higher quality aptamers can be obtained using subtractive SELEX technology. By using two types of cells from homologous sources, one expressing our desired target or phenotype and the other not. The two types of cells are used for alternating negative and positive selection steps. This approach allows to obtain a more specific aptamer sequence, significantly reducing the number of specific binding and selection cycles compared to traditional methods.
Fig 1. Schematic diagram of the subtractive SELEX process [2].
Subtractive SELEX has already had important applications in cancer detection as well as treatment. In the prognosis of hepatocellular carcinoma (HCC), recurrence and metastasis are two key risk factors; therefore, there is an urgent need to develop an early and accurate assay to assess the prognosis of postoperative HCC. Researchers obtained specific aptamers for HCC cells with high metastatic potential by subtractive SELEX technology using two HCC cell lines derived from the same genetic background but with different metastatic potentials.
In the study of gastric cancer, researchers developed four highly specific adapters for gastric cancer sera using the ablative SELEX technique, using gastric cancer sera and normal sera as positive and negative screens, respectively.
With our technology platform, Creative Biolabs provides high-quality aptamer screening services by subtractive SELEX.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs provides the most comprehensive services for aptamer development projects. Our cutting-edge technology allows our researchers to fulfill your requirement from various aspects and to offer the most suitable solution for your project.
References
