During our long history, Creative Biolabs develops and commercializes a full range of integrated platforms and innovative services based on aptamer screening. We offer the CE-SELEX technology platform as one of the methods for rapid and efficient screening of nucleic acid aptamers. We also provide in-house access to qualified aptamers and the CE-SELEX technology platform to assist our customers in their research and project development.
CE-SELEX (capillary electrophoresis) is one of the methods for rapid and efficient screening of nucleic acid aptamers. The technique typically incubates the nucleic acid library with the target protein before separation. Under a high voltage electric field, unbound nucleic acid sequences are separated from nucleic acid-target protein complexes due to different migration rates. The complexes are collected at the exit end of the capillary, amplified and purified by PCR to generate secondary libraries for the next round of screening. CE requires only one to four rounds of screening to obtain aptamers with high affinity and specificity.
| Pre-screening Characterization and Optimization of Binding Conditions |
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| Analysis of Secondary Nucleic Acid Libraries in Screening |
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| Aptamer Affinity Determination After Screening |
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CE has the advantage of online visualization, and the binding strength of protein targets to the initial nucleic acid library can be quickly assessed by visualizing the complex peaks and nucleic acid library peaks, and the kD value can be determined. Based on the CE characteristic electrophoresis map, protein targets can be categorized into high, medium and low affinity classes to establish screening strategies for different target proteins.
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry. If you are interested in this service, please contact us for more details.
