During our long history, Creative Biolabs develops and commercializes a full range of integrated platforms and innovative services based on aptamer screening. We have extensive experience in the field of aptamer screening aptamers with strong affinity for the target molecule can be obtained. We also provide in-house access to qualified aptamers and the AFM SELEX technology platform to assist our customers in their research and project development.
Atomic force microscopy (AFM) allows dynamic detection of the adhesion or affinity between the sample surface and the cantilever. This feature can be used to select aptamers that bind to their targets with very high affinity. By combining SELEX and AFM methods, aptamers with strong affinity for the target molecule can be obtained.
Fig 1. The scheme of a new SELEX strategy for functional oligonucleotide screening by AFM [1].
AFM can work in vacuum, ultra-high vacuum, gas, solution, electrochemical environment, normal temperature and low temperature, so it has a wide range of applications. In biology, atomic force microscopy can be used to study the structure and other properties of biological macromolecules, such as the observation of proteins, RNA, DNA, and even cells and viruses.
In the selection method using AFM, aptamers with high affinity for the target molecule can be obtained in only three replicate rounds. Thus, the AFM SELEX strategy is a very useful method for obtaining aptamers quickly. In this new SELEX strategy using AFM, only the oligonucleotide bound to the target is retained on the chip, while the non-specific binding is reduced on the chip surface, resulting in rapid acquisition of aptamers with high affinity for the target.
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry. If you are interested in this service, please contact us for more details.
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