Creative Biolabs offers a full range of aptamer screening services to customers worldwide, based on state-of-the-art technology and extensive expertise in aptamer development. Based on an advanced technology platform, our expert scientists offer a comprehensive range of aptamer screening services for small molecules from different aspects.
Aptamer is a small in vitro screened oligonucleotide sequence or a short polypeptide that can bind with high affinity and strong specificity to the corresponding target, showing promising applications in many aspects.
Currently, nucleic acid aptamers for protein, cellular and microbial targets are mainly used in disease diagnosis and treatment, drug discovery, proteomics and gene expression regulation studies. Nucleic acid aptamers for small molecule targets such as drugs, amino acids and antibiotics are mainly used in chemical and biosensor recognition as well as food and environmental testing and bioanalysis.
| Molecules with Different Groups | A chemical or biological sensor that recognizes the molecule. |
| Homogeneous Molecules | Aptamer that can recognize a certain type of functional group. |
| Chiral Molecules | Chiral molecule splitting agent. |
Unlike composite targets such as biomolecules, cells and microorganisms, small molecule targets have simple structures, few binding sites and weak affinity to nucleic acids. It is difficult to separate the complexes of small molecule targets and nucleic acids, which makes the screening of small molecule aptamers difficult. Therefore, the screening process of small molecule targets is very different compared with protein targets.
Nucleic acid aptamer screening for small molecules is more difficult than for large molecules, so library selection and design are more important. In addition, nucleic acid libraries have to be optimized to increase affinity properties, such as phosphate modifications. The design of nucleic acid libraries for small molecules can be done with the help of computer software, but not much has been reported yet.
The interaction between target and nucleic acid library in free solution is beneficial to maintain the natural structure and also to simulate the environment of the organism. Therefore, the separation of target-bound nucleic acids in free solution is the most ideal method.
| CE-SELEX | Solid-phase Separation |
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CE-SELEX is one of the methods for rapid and efficient screening of nucleic acid aptamers. In CE-SELEX, the nucleic acid library is usually incubated with the target protein and then CE separation is performed. Under high voltage electric field, unbound nucleic acid sequences are separated from nucleic acid-target protein complexes due to different migration rates. Usually CE-SELEX requires only 1 to 4 rounds of screening to obtain aptamers with high affinity and specificity. |
The solid-phase separation method immobilizes the target or nucleic acid library onto the carrier by chemical bonding, removes and washes the unbound nucleic acids separately by using different solvents, and then dissociates the bound nucleic acids by solvent elution to achieve the purpose of separation. Solid phase separation carrier
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With our technology platform, Creative Biolabs provides high-quality aptamer screening services.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry.
