With extensive experience in aptamer development, our scientists are confident in providing a full range of aptamer screening services to advance science and technology development. Creative Biolabs is able to provide aptamer screening service for chiral molecules in an efficient and high-quality manner.
The demands for separation of chiral compounds are continuously increased in fine chemical/biological and pharmaceutical industry. One of the main concerns in chiral separation is that each enantiomer of a racemic drug may have different pharmacokinetic and pharmacodynamic effects due to their differential metabolism.
Nucleic acid aptamers screened with R- or S-type molecules can bind chiral molecules of that type and distinguish their enantiomers with different spatial structures. Nucleic acid aptamers of chiral molecules can be used as splitting agents for the separation of chiral molecules The nucleic acid aptamer can be used as a splitting agent for the separation of chiral molecules.
Fig 1. Mirror image in vitro selection of cross-chiral DNA aptamers [1].
Nucleic acid aptamers of chiral molecules will have great potential for chiral separation and detection of natural polysaccharides and derivatives, macrocyclic antibiotics, chiral macrocyclic ligands, ligand exchange complexes, and chiral surfactants in the biological and chemical pharmaceutical industries.
Researchers have screened five DNA sequences using isobutylpropionic acid racemate ibuprofen mixed in a 1:1 substance ratio as the target. Three of the DNA sequences showed affinity for both racemate and R-isobutylpropionic acid. The other 2 DNA sequences had affinity for both racemate and S-isobutylpropionic acid. The above five nucleic acid aptamers can be used for the in vivo detection of ibuprofen in pharmacokinetic analysis and the isolation of ibuprofen chiral drugs.
Researchers have immobilized L-tryptophan on agarose microbeads and selected nucleic acid library was firstly reverse screened with L-tyrosine as the target and then interacted with L-tryptophan. DNA nucleic acid aptamers specifically recognizing L-tryptophan were obtained after 12 rounds of screening and could be used for chiral disassembly of D/L-tryptophan.
With our technology platform, Creative Biolabs provides high-quality aptamer screening services for small molecules.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs provides the most comprehensive services for aptamer development projects. Our cutting-edge technology allows our researchers to fulfill your requirement from various aspects and to offer the most suitable solution for your project.
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