With extensive experience in aptamer development, our scientists are confident in providing a full range of in vivo SELEX services to advance science and technology development. Creative Biolabs is able to provide in vivo SELEX related services in an efficient and high-quality manner.
In previous studies, some high-affinity aptamers had been screened by cell-SELEX but failed in subsequent in vivo studies. This is because aptamers are highly specific molecules and any modification to the aptamer target environment will reduce their affinity. Therefore, one of the most important aspects in cell-SELEX is that the conditions and properties of cells used for selection must always be similar to physiological conditions in vivo. In vivo SELEX technology that targets various organs or tissues in the body can solve this dilemma. In vivo SELEX is a technical upgrade of cell-SELEX.
In order to ameliorate the problem of changing the cellular environment faced by cellular SELEX, it is an advantageous option to utilize the whole tissue as a target for tissue-SELEX selection. For example, aptamers that bind to smooth muscle cells are screened in vivo to identify aptamers that bind to the arterial wall.
Tissue SELEX also has a variant, Morph-X-Select, which is based on morphology-based tissue aptamer selection, such as screening for disease-relevant high-affinity aptamers through tissue sections from patients.
Fig. 1 Whole-organism in vivo SELEX [1].
Whole-organism in vivo SELEX is the process of injecting a random library into a living animal or plant and allowing it to circulate throughout the organism. Aptamers are distributed throughout the body, and certain aptamers bind to desired target tissues, while unbound aptamers are eliminated by the kidneys. Afterwards, the target organ/tissue must be removed from the organism for RNA or DNA extraction, and the isolated aptamers are amplified from the target organ/tissue by PCR.
In vivo SELEX is a straightforward and reliable technique for identifying aptamers that selectively recognize diseased organs or tissues in animal models. Using selected aptamers, we can develop nano-based delivery systems with targeting moieties with high efficiency and specificity to overcome particularly robust biological barriers, such as bone and brain, and enable targeted drug delivery.
With our technology platform, Creative Biolabs provides high-quality aptamer screening services by in vivo SELEX.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs provides the most comprehensive services for aptamer development projects. Our cutting-edge technology allows our researchers to fulfill your requirement from various aspects and to offer the most suitable solution for your project.
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