With extensive experience in aptamer development, our scientists are confident in providing a full range of aptamer screening services to advance science and technology development. Creative Biolabs is able to provide aptamer screening services for metal ion in an efficient and high-quality manner.
Heavy metal ions in the environment are difficult to biodegrade and can accumulate thousands of times in the body due to biological amplification in the food chain. In addition, heavy metal ions can affect the activity of proteins and enzymes in the human body and accumulate in some organs, causing chronic toxicity. Mainly Cu, Pb, Zn, Cd, Cr, Hg, As. To detect trace amounts of heavy metal ion contamination in the environment, the generation of nucleic acid aptamers solves this problem.
The excellent optical properties of semiconductor QDs are due to the photoexcitation of the QDs and the transfer of valence-band electrons to the conduction band. Different photophysical fluorescent and chemiluminescence mechanisms, combined with specific recognition of aptamers, are used to develop biosensor systems for proteins and small molecules.
An electrochemical aptasensor was fabricated using an aptamer as a bioreceptor and an electrochemical transducer, which translated the target–aptamer affinity into a measurable electrochemical signal through potentiometry, voltammetry, amperometry, impedimetry, or electrochemiluminescence.
Fig 1. Aptamer-modified semiconductor quantum dots for biosensing applications.
Fig 2. A schematic diagram of SELEX of metal ion [1].
The small molecular weight and low site resistance of heavy metal ions bound to nucleotide sequences will seriously affect their separation in the screening process. Most SELEX methods are not effective in isolating highly specific aptamers. Currently, the commonly used principles for heavy metal ion selection are to immobilize libraries on substrates, including affinity chromatography-SELEX and graphene oxide-SELEX.
| Affinity Chromatography-SELEX | Agarose or resin is filled into the column as a solid matrix, and the oligonucleotide library is immobilized on the solid matrix in the presence of affinity-biotin. Heavy metal ions are injected into the affinity chromatography column as mobile phase for incubation, and some specific nucleic acid sequences are highly attractive to the target through affinity interactions. |
| Graphene Oxide-SELEX | Graphene oxide (GO) is a common separation medium and SELEX based on GO is a common screening method. Using the hydrophobic interaction with base, GO can adsorb ssDNA on surface by π–π stacking. |
Optical aptasensor is an important method for detection of heavy metal ions, including fluorescence aptasensor, colorimetric aptasensor, surface plasmon resonance (SPR) aptasensor, surface enhanced Raman scattering (SERS) aptasensor, fluorescence resonance energy transfer (FRET) aptasensor, chemiluminescence (CL) aptasensor and electrochemiluminescence (ECL) aptasensor.
With our technology platform, Creative Biolabs provides high-quality aptamer screening services.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry.
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