Creative Biolabs offers a full range of aptamer optimization services to customers worldwide, based on state-of-the-art technology and extensive expertise in aptamer development. We customize the best aptamer modification solutions to enhance the stability or specificity of aptamers according to the specific requirements of our customers.
With the rapid development of screening technology, more and more nucleic acid aptamers have been screened and applied to environmental monitoring, gene regulation, drug transport, biosensors and other applications. In these applications, the sequences of the screened nucleic acid aptamers are generally required to be modified to meet the needs of specific applications，and the aptamer sequences obtained from screening are generally required to be modified.
| Truncation | Nucleotide Replacement |
|---|---|
| Aptamer truncation is the reduction of the aptamer length to the minimum sequence required to maintain binding. Truncation increases the affinity of the aptamer because the reduction in sequence length may reduce spatial site resistance or improve the stability of the aptamer tertiary structure. | Nucleotide replacement is a process of point mutation of screened aptamers. The optimized aptamer with the strongest affinity is selected by affinity testing of aptamers mutated at different positions. |
| Replacement / Addition of Non-natural Nucleotides | Aptamer Dimerization / Generation of Bivalent Aptamers |
| The nucleotides of the screened aptamers were replaced with modified nucleotides to improve the performance and in vivo stability of the natural DNA and RNA aptamers. | Combine aptamers with each other to form dimers or bivalent aptamers to increase the affinity. |
| Aptamer Conjugation for Immobilization and Detection | Sequence Cleavage |
| The binding of an aptamer to a biotin is a common technique used to immobilize an aptamer on an affinity-based plate or bead. The immobilized aptamer can be applied with other related techniques. | A strategy to reduce non-specific binding by splitting the nucleic acid aptamer into two fragments and binding them to the target in a ternary complex. |
Fig. 1 The cutting strategy of Sgc8 aptamer [1].
With our technology platform, Creative Biolabs provides high-quality aptamer optimization services.
We committed to help our customers obtain high-quality aptamer optimization services more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry.
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