Creative Biolabs offers a full range of aptamer characterization services to customers worldwide, based on state-of-the-art technology and extensive expertise in aptamer development. Based on an advanced technology platform, our expert scientists offer a comprehensive range of aptamer characterization services from different aspects.
The aptamers obtained after multiple rounds of SELEX technology are not immediately available for therapeutic or drug screening applications. To ensure the efficiency of aptamer applications, these selected aptamers must be highly stable, of moderate length and with good affinity. Therefore, a series of methods including structural analysis, affinity analysis and competition analysis are also required to characterize the aptamers.
| Isothermal Titration Calorimetry (ITC) | ITC can provide detailed information on aptamer KD in the nM to µM range as well as on aptamer binding chemical stoichiometry, enthalpy and entropy. |
| Surface Plasmon Resonance (SPR) | SPR provides not only affinity and chemical metrics, but also characterization of kinetics. |
| Microscale Thermophoresis (MST) | MST is a relatively new technique that can more accurately characterize the KD of small molecule aptamers. |
It is now well established that DNA and RNA aptamers can fold into a myriad of complex architectures, including junctions, bulges, pseudoknots, G-quadruplexes, and triple-stem structures. Determining aptamer structure is another crucial aspect of the characterization process that can provide useful information for engineering aptamer affinity, specificity, and the introduction of additional functionalities useful for sensing purposes.
| Circular Dichroism (CD) | CD spectroscopy is a method that provides information on the structure of an aptamer based on its ability to absorb circularly polarized light to elucidate its possible secondary or tertiary structure. |
| Nuclear Magnetic Resonance (NMR) Spectroscopy | This technique is the most common technique for determining the three-level structure of an aptamer in solution. It is based on the interaction of radiofrequency electromagnetic energy with the nuclei in the host body and the target in a strong magnetic field. |
| X-ray Crystallography | X-ray crystallography can be used to determine the three-dimensional structure of aptamers by exposing a crystalline sample of the aptamer to X-rays and detecting the spatial distribution and intensity of the diffracted rays. |
With our technology platform, Creative Biolabs provides high-quality aptamer characterization services.
We committed to help our customers obtain high-quality aptamers more efficiently. If you are interested in this service, please contact us for more details.
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry.
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