Creative Biolabs offers a full range of aptamer analysis services to customers worldwide, based on state-of-the-art technology and extensive expertise in aptamer development. Based on an advanced technology platform, our expert scientists offer a comprehensive range of aptamer analysis services from different aspects.
Aptamers have been widely used in cellular imaging, new drug discovery, disease treatment and microbial detection, among many other applications. However, the oligonucleotide aptamers screened in vitro are easily degraded in vivo and even exhibit toxicity. Therefore, in vitro analysis is required to evaluate the success of aptamer development after optimization of the screened aptamers.
Cytotoxicity assays are primarily assays based on altered cell membrane permeability and are commonly used in the following ways.
| MTT, XTT | The enzyme activity of the internal mitochondrial enzymes was detected by ELISA. |
| LDH | Cytotoxicity is detected by measuring the enzymatic activity of lactate dehydrogenase in cell culture supernatants. |
| Other Enzymatic Methods | Detection of alkaline phosphatase and acid phosphatase activity in the supernatant, etc. |
| Cell Proliferation Capacity Analysis Kit | The OD value is read by enzyme marker, thus detecting the cell value-added status. |
| Fluorescein Luminescence | When cells are damaged, the cell membrane is broken and AK is released into the culture supernatant. Using luciferase and luciferin in the presence of ATP can be luminescent and can be detected quantitatively by chemiluminescence. |
Creative Biolabs is a leading global aptamer development company. After more than a decade of exploration and expansion, we have demonstrated solid expertise and capabilities in biotechnology and have expanded our research and service capabilities to include all areas of aptamer development.
Our commitment to this long-term goal has driven us to provide the highest quality services to our customers in order to further advance the biotechnology/pharmaceutical industry.
